Skip to main content
. 2012 Sep 17;7(9):e45102. doi: 10.1371/journal.pone.0045102

Figure 2. Binding of CD11c-scFv-IDRgag to bmDCs and spleen DCs.

Figure 2

(A) Binding of CD11c-scFv-IDRgag to bmDCs at different concentrations. Data represent mean±SD values of 4 independent experiments. (B) At 10 µg/ml concentration, CD11c-scFv-IDRgag bound to bmDCs with significantly higher capacity as compared to the control-scFv-IDRgag. Data represent mean±SD values of 4 independent experiments. Data were analyzed with unpaired t-test. (C) Using spleen cell suspensions, we investigated binding-specificity of CD11c-scFv-IDRgag on DCs. By gating on CD11b and MHC class II we defined a population of CD11c expressing DC without staining for CD11c itself. Whereas CD11b+/MHC class II- cells did not express CD11c, the CD11b/MHC class II double-positive population represented CD11c expressing DCs. We found that CD11c-scFv-IDRgag selectively targets CD11c positive cells in vitro. Results of one representative experiment are shown.