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. 2012 Sep 17;7(9):e45102. doi: 10.1371/journal.pone.0045102

Figure 4. Targeting antigens to CD11c on DCs improved activation of specific T cells measured by up-regulation of CD69 and CD25 in vitro.

Figure 4

(A) Activation of FV-specific TCRtg CD8+ T cells upon co-culture with DCs loaded either with C-opsonized F-MuLV (F-MuLV-C), 10 µg/ml CD11c-scFv-IDRgag or 10 µg/ml control-scFv-IDRgag. Data display one representative experiment showing the percentage of CD69 expressing CD8+ T cells. Co-expression of CD69 and CD25 either on FV-specific CD8+ T cells upon co-culture with DCs loaded either with C-opsonized F-MuLV (F-MuLV-C), 10 µg/ml CD11c-scFv-IDRgag or 10 µg/ml control-scFv-IDRgag (B). Co-expression of CD69 and CD25 either on FV-specific CD4+ T cells after 24 hours co-culture with differentially loaded DCs (D). Data represent mean±SD of 8 and 3 independent experiments for CD8+ T cell and CD4+ T cell co-cultures, respectively. Data were analyzed with unpaired t-test. Co-expression of CD69 and CD25 on OVA-specific CD8+ OT-1 (C) or CD4+ OT-2 (E) T cells after 24 hours co-culture with differentially loaded DCs. Data represent mean±SD of 3 and 4 independent experiments for CD8+ T cell and CD4+ T cell co-cultures, respectively. Data were analyzed with unpaired t-test.