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. 2012 Sep 17;7(9):e45272. doi: 10.1371/journal.pone.0045272

Figure 4. Th1 and Th17 cells induce PD-L1 expression on MSC.

Figure 4

(A) Percentage of MSC expressing PD-L1 after co-culture with activated T cells undergoing Th1 or Th17 lineages (CD4-Th1 or CD4-Th17, respectively) or (B) with mature activated Th1 or Th17 cells, detected by flow cytometry. (C) PD-L1 molecule expression profile on MSC after 6 hours of incubation with Th1- or Th17-conditioned media. (D) The percentages of MSC positive for PD-L1 and the mean fluorescence intensities (MFI) upon a 6 hours treatment with IL-17 and IFN-γ alone or in combination with TNF-α and IFN-γ. On the right, representative flow cytometry dot plots illustrating PD-L1 protein expression on untreated control and treated MSC. If not indicated, P values referred to the condition corresponding to MSC alone.