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. 2012 Sep 17;7(9):e45272. doi: 10.1371/journal.pone.0045272

Figure 6. PD-L1 blockade partially reverse the immunosuppressive properties of MSC on mature Th17 cells.

Figure 6

We assessed the effect of MSC on the proliferation and differentiation of activated CD4+ T cells induced to differentiate into (A and C) Th1 (CD4-Th1) and (E and G) Th17 (CD4-Th17) cells and on mature (B and D) Th1 and (F and H) Th17 cells in the presence or absence of 10 µg/ml of neutralizing antibodies against PD-L1 or PD-1. We used the Cell Titer GloTM luminescence assay to quantify T cell proliferation and flow cytometry intracellular detection of IFN-γ (Th1) or RORγT and IL17 (Th17) for differentiation. Proliferation results are expressed as the percentage of anti-CD3/CD28-induced proliferation of the different T cell subsets which was assigned the value of 100%. If not indicated, P values referred to values obtained for MSC when cultured alone (white bars). CD4+ T cells undergoing Th1 lineage and mature Th1 cells are represented with black bars. CD4+ T cells undergoing Th17 lineage and mature Th17 cells are represented with grey bars.