Table 5. Clinical performances of the 3 best molecules for discriminating early (blood sampling between 0 and 3 h after symptoms onset, N = 22) vs. late stroke patients (blood sampling strictly after 3 h, N = 81).

		GST-π	NDKA	DJ-1
	AUC (95%CI)	0.79*(0.69–0.89)	0.68 (0.57–0.79)	0.66 (0.54–0.78)
Youden index	CO (ug/L)	39.6	4.4	60.3
	Se/Sp (%)	68.2/82.5	91.0/44.0	86.4/50.0
	OR	10.1	8.0	6.4
	NPV/PPV (%)	90.4/51.7	94.7/30.8	93.0/32.2
High Se	CO (ug/L)	17.7	4.4	50.8
	Se/Sp (%)	90.9/50.0	91.0/44.0	90.9/42.5
	OR	10.0	8.0	7.4
	NPV/PPV (%)	95.2/33.3	94.7/30.8	94.4/30.3
High Sp	CO (ug/L)	65.2	23.1	443.0
	Se/Sp (%)	50.0/91.2	18.2/90.1	18.2/97.5
	OR	10.4	2.0	8.6
	NPV/PPV (%)	86.9/61.1	80.2/33.3	81.2/66.7

NPV: negative predictive value/PPV: positive predictive value.

^*The AUC of GST-π was significantly better than AUC of NDKA and DJ-1 (p = 0.034 and 0.020 respectively). No significant difference was obtained between AUC of NDKA and of DJ-1 (p = 0.63).

An AUC above 0.74 was estimated as significant (significance 0.05 and power 0.95, made with Power tests/Sample size item from pROC software).