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. 2012 Sep 17;7(9):e45031. doi: 10.1371/journal.pone.0045031

Figure 6. Aid shuttling is associated with Aid-mediating transcloation of Tet1.

Figure 6

(A) A schematic representation of the Aid structure and its mutants used in this study. All Aid constructs were tagged with C-terminal Myc. (B–D) The upper figures are representative confocal images of DLD-1 cells transiently expressing only Aid FL (B), ΔNES (C) or F193A (D). The lower figure represents the proportion of cells with different subcellular localization of Aid. Aid mutants defect in NES showed the increased nuclear localization. *p<0.05 vs Aid FL. (E, F) The upper figures are representative confocal images of DLD-1 cells transiently co-expressing AidΔNES and Tet1CD (E), or AidF193A and Tet1CD (F). Tet1CD were tagged with N-terminal Xpress. The lower figure shows the proportion of cells with different localizations of Tet1 (E; AidΔNES and Tet1CD, F; AidF193A and Tet1CD). Aid mutants, which exhibit impaired shuttling between the nucleus and the cytoplasm, failed to alter the subcellular localization of Tet1. #p<0.05 vs with Aid FL. (G) The upper figures are representative confocal images of DLD-1 cells transiently expressing only AidΔN26. The lower figure represents the proportion of cells with different subcellular localization of AidΔN26. *p<0.05 vs Aid FL. (H) The upper figures are representative confocal images of DLD-1 cells transiently co-expressing AidΔN26 and Tet1CD. The lower figure shows the proportion of cells with different localizations of Tet1. #p<0.05 vs with AidFL. The scale bars are 10 µm. N (black); nuclear localization, N+C (gray); both nuclear and cytoplasmic localization, C (white); cytoplasmic localization in multiple microscope fields.