TABLE 3.
Characterization and complementation of P. aeruginosa strains isolated from intubated patients
| Strain (genotype) | Productiona of:
|
Productionb of:
|
Elastaseb (OD495) | Productione of:
|
Adhesionb (%) | Biofilmb (%) | |||
|---|---|---|---|---|---|---|---|---|---|
| C4-HSL (μM) | 3-Oxo-C12-HSL (nM) | Pyocyanin (μg/ml) | HCN (μM) | Rhamnolipids | Proteases | ||||
| PAO1 | 5 | 300 | 10 | 29.2 | 2.7 | +++ | +++ | 100 | 100 |
| 4.1A1 (L) | 5 | 250 | 1 | 52.8 | 2.7 | +++ | +++ | 97 | 88 |
| 3D10 (C) | 4.5 | 340 | 8.9 | 23.4 | 2.7 | + | ++ | 104 | 91 |
| 7B5 (B) | 3 | 125 | 12 | 15.3 | 2.7 | + | +++ | 147 | 150 |
| 19G12 (H) | 1 | 260 | 3.5 | 27.6 | 2.3 | + | +++ | 31 | 10 |
| 36D5 (A) | 3 | 340 | 5.1 | 43.6 | 1.2 | ++ | +++ | 130 | 76 |
| 60B5 (D) | 4 | 375 | 8 | 34.3 | 2.6 | + | +++ | 139 | 33 |
| 22D10 (F) | <0.5 | <50 | 2.5 | <5 | 0.03 | BD | + | 62 | 70 |
| lasR+ | 2 | 150 | 25 | 9.7 | 2.6 | +++ | +++ | NDd | ND |
| rhlR+ | <0.5 | <50 | 2.3 | <5 | 1.1 | BD | + | ND | ND |
| 1.1A1 (K) | <0.5 | <50 | <1 | <5 | BDc | BD | BD | 116 | 47 |
| lasR+ | 1 | 375 | <1 | 9.2 | 2.7 | BD | +++ | ND | ND |
| rhlR+ | <0.5 | <50 | 27.8 | <5 | 0.8 | + | + | ND | ND |
| 18F7 (I) | <0.5 | <50 | <1 | <5 | BD | BD | BD | 88 | 76 |
| lasR+ | 4 | 300 | <1 | 9.4 | 1.2 | BD | ++ | ND | ND |
| rhlR+ | <0.5 | <50 | 27.4 | <5 | 0.6 | + | + | ND | ND |
a
Values are means of three independent experiments with a standard deviation of 10%.
b
Values are means of three independent experiments with a standard deviation of <10%.
c
BD, below detection.
d
ND, not done.
e
+++, high level; ++, intermediate level; +, low level.