Table 1.
Sf double mutant trafficking receptor gene expression and its effect on skin and lung inflammation: Lack of skin and lung inflammation in Sf.II2−/− mice correlates with lack of increased expression of Th2 related trafficking receptor genes. Liver inflammation is representative of systemic inflammation that includes inflammation in the colon, pancreas and salivary glands.*
| Strain | TRG expression | Liver inflammation | Lung inflammation | Skin inflammation | |
|---|---|---|---|---|---|
| Th1-related | Th2-related | ||||
| B6 | 1 | 1 | 0 | 0 | 0 |
| Sf** | 3 | 3 | 3 | 4 | 4 |
| Sf.Il2−/− | 3 | 1 | 3 | 1 | 0 |
| Sf.Il4−/− | 4 | 2 | 4 | 4 | 4 |
| Sf.Ifng−/− (6 wk) | 2 | 3 | 3 | 3 | 3 |
| Sf.Stat6−/− | 4 | 2 | 4 | 4 | 4 |
| Sf.Itgae−/− | 3 | 3 | 3 | 2 | 1 |
| Sf.Ltb4r1−/− | 3 | 3 | 3 | 3 | 2 |
| Sf.Alox5−/− | 3 | 4 | 4 | 4 | 4 |
| Sf.Il10−/− | ND | ND | 4 | 4 | 4 |
| Sf.Cx3cr1gfp/gfp | ND | ND | 3 | 4 | 4 |
| Sf.Fas−/− (12 wk) | ND | ND | 3 | 4 | 4 |
Trafficking receptor gene expression was determined by quantitative PCR and microarray assays [4,6–7]. Inflammation of individual organs was determined by histology and visual examination of the skin. Mice are examined at 3–4 weeks of age. ND: not determined. An arbitrary assignment of “1” for trafficking receptor expression is given to B6 mice as a reference..
Sf, Scurfy and Fox P3 deficient; Ifng, Interferon-γ; Itgae, integrin αE; Ltb4r1, leukotriene B4 receptor 1; Alox5, arachidonate 5-lipoxygenase; Cx3cr1gfp/gfp, Cx3cr1 deficient and expression of EGFP under control of endogenous Cx3cr1 locus.