Figure 1. Experimental design.
Scaffolds were sorted according to the mass density into three experimental groups (low, medium and high density) and their structural and mechanical properties were characterized. Medium density scaffolds were demineralized to assess the effect of matrix mineralization on osteogenesis. Human mesenchymal progenitors derived from hESC were characterized fir their phenotype and multipotency, seeded into scaffolds, and cultured for 5 weeks.