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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biomaterials. 2012 Aug 16;33(33):8329–8342. doi: 10.1016/j.biomaterials.2012.08.013

Figure 3. Mesenchymal progenitors.

Figure 3

Flow cytometry data demonstrated lack of expression of hESC (SSEA-4), early differentiating cells (SSEA-1), endothelial cells (CD31), hematopoietic cells (CD34) and neural cells (CD261) specific markers. Positive expression of mesenchymal markers was detected in mesenchymal progenitors derived from hESC line H9 (a). Mesenchymal progenitor potential for osteogenesis was demonstrated by positive staining of alkaline phosphatase activity (purple) in monolayer cultures (scale bar: 100μm) (b). Pellet cultures indicated osteogenic and chondrogenic potential under adequate conditions, as shown by black von Kossa staining (matrix mineralization, scale bar: 500μm) and blue Alcian Blue staining (GAG) (scale bar: 250μm), respectively (c).

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