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Reconstructed 3D μCT images of the engineered bone before and after 5 weeks of culture (scale bar: 1 mm) (a). Bone volume (BV) (b), bone volume to tissue volume fraction (BV/TV) (c), trabecular number (Tb.N.) (d), intertrabecular space (Tb.Sp.) (e), and trabecular thickness (Tb.Th.) (f) were determined by μCT analysis, and indicated bone maturation during in vitro culture. Data represent average ± SD, n=4 (*p<0.05; **p<0.01; ***p<0.001).
