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Determination of the apparent cellular half-life of WT and mutant hGK forms. (A–F): Semi-logarithmic plot of the time course of the degradation of WT, S263P and G264S mutant forms (data from Fig. 3). Linear regression analysis revealed a biphasic time course for WT hGK in HEK293 (A) and MIN6 cells (B), whereas a monophasic time course was observed for the S263P mutant in HEK293 (C) and MIN6 (D) cells, and the G264S mutant in HEK293 (E) and MIN6 (F) cells.
