Skip to main content
. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jul 20;1822(11):1705–1715. doi: 10.1016/j.bbadis.2012.07.005

Fig. 8.

Fig. 8

Thermal unfolding of WT and G264S hGK. The thermal unfolding profile of WT (A) and G264S (B) at a concentration of 10 μM in the absence of Glc was obtained by following the change in ellipticity at 222 nm at a constant heating rate of 40 °C · h−1. From the first derivative of the smoothed denaturation curve, apparent transition temperatures of ~40.8 °C (A) and ~35.5 °C (B) were determined for the WT and G264S hGK, respectively.