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. Author manuscript; available in PMC: 2013 Sep 14.
Published in final edited form as: Mol Cell. 2012 Jul 19;47(5):767–776. doi: 10.1016/j.molcel.2012.06.016

Fig. 3. Protein Oxidation during the Lifespan of WT, daf-2, and daf-16 Worms.

Fig. 3

Relative distribution of protein thiol oxidation in synchronized populations of wild-type N2, daf-2 and daf-16 mutant strains during development (L4) and early adulthood (day 2). Protein oxidation was determined using OxICAT (complete list of proteins and their oxidation states at time points L2, L4, Day 2, 8 and 15 can be found in Table S2). Shown is the frequency distribution of average oxidation levels determined for all of the protein thiols that were reproducibly identified for each time point. The histograms were fitted to a single or double Gaussian model (red line) using GraphPad Prism, with R2 values of 0.8–0.9. Statistical analysis was done using Kruskal-Wallis one-way analysis of variance followed by Dunn’s post test. p values <0.05 are indicated with *; p values from 0.001 to 0.01 are indicated with **.