Figure 9.

Cell-based selection of RNA aptamer against mouse embryonic stem cells (mESCs; Iwagawa et al. 2011). (A) SELEX schematic of live mESCs combined with counterselection against fully differentiated A-9 cells. (B) Consensus motif conserved in the anti-mESC aptamer L2-2. N, K, Y, and R indicate any 4, G or U, C or U, and A or G nucleotides, respectively. (C) Confocal fluorescence image of the L2-2 aptamer. mESCs before and after treatment for rheumatoid arthritis for 4, 8, or 14 days were stained with the indicated fluorescein-labeled RNA probes and APC-labeled antibodies. DIC images are also shown. Scale bar, 50 μm.