Fig. 5.

ERp57 is involved in integrin mediated adhesion and clot retraction. Microtitre plates were coated with (A) collagen (20 μg mL⁻¹) or (B) fibrinogen (1 mg mL⁻¹). Platelets pre-treated with F(ab)′ fragment of IV.3 antibody and then incubated with either pre-immune IgG (37.5 μg mL⁻¹) or anti-ERp57 (12.5–37.5 μg mL⁻¹) were allowed to adhere. Adherent cells were lysed with acidic lysis buffer and the assay developed using p-NPP substrate. Absorbance data represent 4 separate experiments, **P < 0.01, ***P < 0.005. Clot retraction was measured using human PRP adjusted to 4 × 10⁸ cells mL⁻¹. Cells were incubated with either pre-immune IgG (37.5 μg mL⁻¹) or anti-ERp57 (12.5–37.5 μg mL⁻¹) for 5 min. (C) Clots formed in the presence of varying concentrations of anti-ERp57 after 2 h and (D) mean data for clot weight. n = 4, *P < 0.05, **P < 0.01.