Fig. 5.

Biotinylated immunoglobulin (Ig)G isotype control binding on leukocyte populations. Human IgG, mouse IgG and infliximab were biotinylated. Binding of each IgG class to the cell surface of leucocyte populations was determined using healthy control (HC) blood. The lymphocyte, monocyte and granulocyte populations were gated on forward/side scatter (FSC/SSC), as depicted in Fig. 1. Each histogram represents streptavidin-phycoerythrin (PE) binding to IgG in each cell population. Data were analysed using BD FACSDiva version 5.0.2 (BD Biosciences, Oxford, UK).