Figure 6.
Dynein depletion inhibits mTORC1 activity. HFs were infected with lentiviral vectors expressing shRNA specific for luciferase (Luc), a control, or dynein heavy chain (Dyn) for 24 h followed by mock or HCMV infection. Seventy-two hours post HCMV infection, cells were lysed and proteins were separated by SDS-PAGE followed by Western analysis for total 4E-BP (T-4E-BP1), phospho-4E-BP (P-4-EBP1 37/46), dynein, and actin.