Figure 2.
Mitochondrial phenotypes in Hippo pathway mutant backgrounds. (A–L) Third instar wing discs. (A,B) Isolated mutant clones of wts (green) generated using MARCM and stained for ATP-syn (red in B) show increased staining in the mutant (green) cells. (C–H) Third instar wing discs containing mutant clones (nongreen) for warts (wts) (C,D), fat (ft) (E,F), and hippo (hpo) (G,H) show increased mitochondrial staining using ATP synthase-α antibody (red) in the mutant cells (nongreen) compared with adjacent wild-type tissue (green). (B,D,F,H) Red channel alone is shown for clarity. Bar, 50 μm. (I,J) Clones of cells in a wing disc expressing Yki (green) using the Ay-Gal4 system (see the Materials and Methods). Increased expression of the mitochondrial marker ATP synthase-α (red) is seen in Yki overexpressing cells (green) as compared with the adjacent wild-type cells (nongreen). (J) Red channel alone is shown for clarity. Bar, 5 μm. (K,L) Knockdown of wts function in the dorsal compartment of the wing disc (K; green, ap-Gal4 UAS-GFP, UAS-wtsRNAi) causes an increase in mitochondrial marker ATP synthase-α staining (L; red). The white dotted line marks the dorsal–ventral boundary. Bar, 50 μm. (M–P) Pupal eye discs. (M,N) Control, pupal eye disc from spa-Gal4, UAS-GFP background. The cone cells are marked with GFP (M; green) and stained for mitochondrial marker ATP-syn (N; red). (O,P) Overexpression of Yki in cone cells (spa-Gal4, UAS-GFP, UAS-yki) (O; green) causes a significant increase in mitochondrial marker ATP-syn staining (P; red). Bar, 5 μm. (Q–T) Clones of cells in the fat body expressing Yki (shown in Q and S in green) using the Ay-Gal4 system (see the Materials and Methods). Increased staining with ATP synthase-α antibody (R; red) and increased uptake of the mitochondrial membrane potential-sensitive dye MitoTracker Red (S; red) can be seen in Yki overexpressing cells (S; green) at the perinuclear regions when compared with adjacent wild-type cells (S; nongreen). Bars, 5 μm.