Table 3.
Diagnostic accuracy of NS-1 antigen detection, rRT-PCR, and IgM antibody detection on acute plasma specimens
| Tests | Paired serology result |
Sensitivity % (95% CI) | Specificity % (95% CI) | PPV % (95% CI) | NPV % (95% CI) | |||
|---|---|---|---|---|---|---|---|---|
| Dengue | Not dengue | |||||||
| Individual assays | NS-1 | + | 39 | 0 | 54.2 (42.0-66.0) | 100 (96.0-100) | 100 (91.0-100) | 73.2 (64.4-80.8) |
| − | 33 | 90 | ||||||
| rRT-PCR | + | 64 | 4 | 88.9 (79.3-95.1) | 95.6 (89.0-98.8) | 94.1 (85.6-98.4) | 91.5 (83.9-96.3) | |
| − | 8 | 86 | ||||||
| IgM | + | 12 | 11 | 16.7 (8.9-27.3) | 87.8 (79.2-93.7) | 52.2 (30.6-73.2) | 56.8 (48.2-65.2) | |
| − | 60 | 79 | ||||||
| Combined assays | NS-1+rRT-PCR | + | 67 | 4 | 93.1 (84.5-97.7) | 95.6 (89.0-98.8) | 94.4 (86.2-98.4) | 94.5 (87.6-98.2) |
| − | 5 | 86 | ||||||
| NS-1+IgM | + | 43 | 11 | 59.7 (47.5-71.1) | 87.8 (79.2-93.7) | 79.6 (66.5-89.4) | 73.1 (63.8-81.2) | |
| − | 29 | 79 | ||||||
| rRT-PCR+IgM | + | 66 | 15 | 91.7 (82.7-96.9) | 83.3 (74.0-90.4) | 81.5 (71.3-89.2) | 92.6 (84.6-97.2) | |
| − | 6 | 75 | ||||||
| NS-1+rRT-PCR+IgM | + | 67 | 15 | 93.1 (84.5-97.7) | 83.3 (74.0-90.4) | 81.7 (71.6-89.4) | 93.8 (86.0-97.9) | |
| − | 5 | 75 | ||||||
rRT: reverse transcriptase real-time; PPV: positive predictive value; NPV: negative predictive value.