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Bacterial complementation assays to demonstrate DHFR and TS functions of PoDHFR-TS coded sequence. (A) E. coli BL21 (DE3) transformed with either pET17b or pET17bPoDHFR-TS was grown on MM in the absence or presence of 4 μM TMP. (B) E. coli χ 2913recA-DE3 carrying pET17b or pET17bPoDHFR-TS was cultured on MM with and without 50 μg/ml thymidine. MM used in A and B also contained 100 μg/ml ampicillin.
