Fig. 3.

Coomassie blue-stained SDS-PAGE and X-ray film exposed to [³H] FdUMP labeling of recombinant PoDHFR-TS. Lane M, molecular weight markers; (1) uninduced cells carrying pET17b; (2) induced cells carrying pET17b; (3) uninduced cells carrying PoDHFR-TS gene; (4) induced cell carrying PoDHFR-TS gene; (5) insoluble fraction of (4); (6) soluble fraction of (4); (7) purified PoDHFR-TS after MTX-affinity chromatography; (8–10) purified PoDHFR-TS, PvDHFR and TcDHFR-TS; (11–13) autoradiographs of (8–10) following [³H] FdUMP labeling.