. 2012 Jun;61(2):324–332. doi: 10.1016/j.parint.2011.12.004

Table 1.

Oligonucleotides used to perform site-directed mutagenesis to correct the sequence of cloned podhfr-ts.

Corrected mutation	Sense (F) and antisense (R) primers used
I225T	F; GTAGTAACGGAACCACATTAGACTTTCTAATTTA
I225T	R; TAAATTAGAAAGTCTAATGTGGTTCCGTTACTAC
236K	F; TTACAGTAGGAAAAAAAAAGACACACAAAATG
236K	R; CATTTTGTGTGTCTTTTTTTTTCCTACTGTAA
S258T	F; GCTACAGCTACAGCGACATGGCCAGGAATAG
S258T	R; CTATTCCTGGCCATGTCGCTGTAGCTGTAGC
R303W	F; GGAGCAGAATGGTGGGGAAATATGGCT
R303W	R; AGCCATATTTCCCCACCATTCTGCTCC
H476Y, D478N	F; GCTGAATATACAAATATGTATGATAATTATGAAAATAAAGGAATTG
H476Y, D478N	R; CAATTCCTTTATTTTCATAATTATCATACATATTTGTATATTCAGC

Corrected nucleotides are underlined.