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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Acta Trop. 2012 Jul 3;124(1):79–86. doi: 10.1016/j.actatropica.2012.06.010

Fig. 2.

Fig. 2

Identification of T. cruzi DTUs by PCR strategies.

A) Amplification of the intergenic region of the mini-exon genes with primers TCC/TC2, lane (1) reference stock Tu 18 (TcII); lane (2) reference stock M5631 (TcIII); lane (3) reference stock Can III (TcIV); lane (4) reference stock PAH 263 (TcV); lane (5) reference stock CL-Brener (TcVI); lane (6) D. novemcinctus; lane (7) reference stock CA-1 K98 (TcI); lane (8–9) D. albiventris; lane (10) 100 bp ladder. B) Amplification of the intergenic region of the mini-exon genes with primers UTCC/TCac, lane (1) 100 bp ladder; lane (2) reference stock Tu 18 (TcII); lane (3) reference stock PAH 263 (TcV); lane (4) reference stock CL-Brener (TcVI); lane (5) reference stock Can III (TcIV); lane (6) reference stock M5631 (TcIII); lane (7) D. novemcinctus; lane (8) reference stock CA-1 K98 (TcI); lane (9–10) D. albiventris.

C) Amplification of the D7 ribosomal DNA 24S-alpha domain with primers D71/D76, lane (1) reference stock Tu 18 (TcII); lane (2) reference stock Can III (TcIV); lane (3) reference stock PAH 263 (TcV); lane (4) reference stock CL-Brenner (TcVI); lane (5) 100 bp ladder; lane (6) reference stock CA-1 K98 (TcI); lane (7–8) D. albiventris; lane (9) reference stock M5631 (TcIII); lane (10) D. novemcinctus.