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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Cell Host Microbe. 2012 Sep 13;12(3):313–323. doi: 10.1016/j.chom.2012.07.012

Figure 2. Detection of arginine metabolites by mass spectrometry (MS).

Figure 2

(A and B) BMDMs were untreated (-) or stimulated with LPS + IFN-γ or IL-4 for 24 hours, and cell lysates were analyzed by MS for arginine (Arg), citrulline (Cit), argininosuccinate (Arg-Succ), and ornithine (Orn). Sample mass spectra are shown (A) with the corresponding concentrations of indicated metabolites (B).

(C-F) BMDMs were infected with BCG with or without IFN-γ in the presence of heavy 13C-arginine (C, D) or 15N-arginine (E, F) for 24 hours, and cell lysates were analyzed by MS as above. The right y-axis was used for MS analysis of argininosuccinate (C, E). Data are representative of at least 3 experiments (A, B) or one experiment (C-F) with BMDMs pooled from at least 4 mice. Recycled arginine (Re-Arg).

Error bars, SEM.

See also Figure S2.