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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Cell Host Microbe. 2012 Sep 13;12(3):313–323. doi: 10.1016/j.chom.2012.07.012

Figure 3. Citrulline is exported from macrophages following iNOS-mediated arginine metabolism.

Figure 3

(A and B) BMDMs (n=3) were untreated (-) or stimulated with LPS + IFN-γ or IL-4 in the presence of 3H-arginine for 24 hours. Levels of arginine and its metabolites were determined by thin layer chromatography (TLC) followed by scintillation counts per minute (cpm). A sample TLC is shown (A) with the corresponding relative amounts of intracellular and extracellular arginine and citrulline (B). Note the difference in scale from the cell lysate and supernatant graphs. Data are the mean cpm.

(C-E) BMDMs (3 mice pooled) were stimulated with LPS + IFN-γ or BCG + IFN-γ in the presence of 3H-arginine over time. Extracellular levels of arginine (C) and citrulline (D and E) were determined by TLC as above. NO production was determined by assaying supernatant NO2- (D and E).

Error bars, SD.

See also Figure S3.