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Evaluation of the LightCycler PCR assay with two representative strains of Coccidioides posadasii. Results of the melting curve analysis are shown of a strain (wild type, wt) with cytosine at position 1228 in accordance with the GenBank sequence of antigen 2/proline-rich antigen (accession number AF013256) and a strain with a mutation to guanosine at this nucleotide position, shifting the melting point to 57°C.
