TABLE 1.
Oligonucleotide primers and LightCycler hybridization probes used in the PCR assays
| Oligonucleotide | Sequence (5′-3′)a | Target gene | Nucleotide positions | GenBank accession no. | Reference |
|---|---|---|---|---|---|
| Cocci I | GTA CTA TTA GGG AGG ATA ATC GTT | Proline-rich antigen | 841-864 | AF013256 | This study |
| Cocci II | GGT GTC AAC TGG TGG GAT GTC AAT | Proline-rich antigen | 1366-1343 | AF013256 | This study |
| Cocci III | ATC CCA CCT TGC GCT GTA TGT TCG A | Proline-rich antigen | 962-986 | AF013256 | This study |
| Cocci IV | GGA GAC GGC TGG ATT TTT TAA CAT G | Proline-rich antigen | 1303-1279 | AF013256 | This study |
| Cocci-HP-1 | CCA AAT TCT TGC ATC TCG CCC A-[FL] | Proline-rich antigen | 1209-1230 | AF013256 | This study |
| Cocci-HP-2 | [Red 640]-ATG GGA TAA GAT GAG AAG ATG GAA AG-Ph | Proline-rich antigen | 1234-1259 | AF013256 | This study |
| GAC.1 | ATG TCTCGC CTG GAG CAC | GAC microsatellite-containing locus | Fisher et al. (12) | ||
| GAC.2 | GCT GTT CCA CGT TTC GAC | Fisher et al. (12) | |||
| 621.1U | ACA ATG AAC GAG CAG CAA GG | 621 microsatellite-containing locus | Fisher et al. (12) | ||
| 621.2L | TGA AAG ATG TGT AGA CCC GA | Fisher et al. (12) |
a
FL, fluorescein; Red 640, LightCycler Red 640-N-hydroxysuccinimide ester; Ph, 3′-phosphate.