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. 2004 Feb;42(2):639–644. doi: 10.1128/JCM.42.2.639-644.2004

FIG. 1.

FIG. 1.

Size variation of PCR products of prtF1 among the 77 GAS isolates (A) and HaeIII restriction profiles obtained from the PCR products of the 46 isolates whose amplicons were digested by HaeIII (B). (A) Lane 1, amplicon size of 127 bp, suggesting the presence of a single RD2 repeat (n = 18); lane 2, amplicon size of 238 bp, suggesting the presence of two RD2 repeats (n = 1); lane 3, amplicon size of 349 bp, suggesting the presence of three RD2 repeats (n = 27); lane 4, amplicon size of 460 bp, suggesting the presence of four RD2 repeats (n = 30); lane 5, amplicon size of 571 bp, suggesting the presence of five RD2 repeats (n = 1). (B) Lanes 1 to 3, restriction profiles obtained from strains with three RD2 repeats (lane 1, three bands of 59, 111, and 179 bp; lane 2, three bands of 59 and 68 bp [comigrating] and 222 bp; and lane 3, two comigrating bands of 170 and 179 bp); lanes 4 and 5, restriction profiles obtained from strains with four RD2 repeats (lane 4, two bands of 170 and 290 bp, and lane 5, two bands of 59 and 401 bp); lane 6, restriction profile obtained from the single strain with five RD2 repeats (three bands of 59, 222, and 290 bp). Lane MW, DNA size markers.