FIG. 2.

RD2 types and organization of the RD2 region in the 77 prtF1-positive GAS based on PCR and restriction enzyme cleavage analysis of PCR products. The organization of the RD2 region of GAS strains was outlined on the basis of the results of RD2 typing and the published sequence of a five-repeat prtF1 gene (28). Superscript letters: a, The reported sizes of PCR products include the primers, which are known to partially overlap the ends of RD2 by eight (5′ primer) or nine (3′ primer) nucleotides (28). nr, not restricted by HaeIII; b, Light shading, 96-bp repeat; dark shading, 111-bp repeat; hatching, primer. Vertical bars indicate restriction sites (Ha, HaeIII; Dd, DdeI; and Hi, HinfI) within individual repeats.