Figure 6.

Effects of antioxidants without a thiol-group, vitamin E (A) and melatonin (B), on 15d-PGJ₂-induced cell death. Cells were precultured for 24 h at 5× 10³ /well in 96-well plates and exposed to 15d-PGJ₂ in the presence or absence of vitamin E (1 μM) or melatonin (1 mM) for 24 h. Cell viability was assessed by fluorescent assay, and data represent the mean ± S.D. from 4 to 5 independent preparations. Statistical significance was assessed by t-test.