(A) Multiplex PCR with mtDNA prepared from causative agents of taeniasis or cysticercosis. Lanes 1 to 10, T. saginata; lane 11, a mixture of T. saginata and T. asiatica; lanes 12 to 16, T. asiatica; lanes 17 to 25, American/African genotype of T. solium; lanes 26 to 32, Asian genotype of T. solium. The lane numbers correspond to the lanes shown in Table 1. (B) Confirmation by multiplex PCR of cysticerci developed in immunodeficiency mice. The cysticerci originated from the eggs in lane 11 of panel A. Lanes 1 to 4, 6 to 8, 10, 12, 13, and 16, T. asiatica; lanes 5, 9, 11, 14, and 15, T. saginata. M, DNA size markers (100-bp ladder [Promega]).