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. 2012 Sep;169(3):281–291. doi: 10.1111/j.1365-2249.2012.04613.x

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© 2012 The Authors. Clinical and Experimental Immunology © 2012 British Society for Immunology

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Fig. 3 — Type 1 CD4⁺ T cell response in infants and adults. (a) Flow cytometry dot-plots show co-expression of early T cell activation marker CD69, with various individual cytokines [tumour necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL-2)] among infants and adults that were gated previously on CD4⁺ T cells, as described in Fig. 1a. Frequencies of various T helper type 1 (Th1) cytokine combinations generated with Boolean gating were normalized with total CD4⁺ T cells in the respective samples, background-corrected with unstimulated controls and then presented as a bar graph (described in Methods) Open triangles represent infants while closed triangles represent adults. (b) or pie charts (c) & (d). The bar graphs show mean percentages of CD4⁺ T cells (all IL-4^-) expressing IFN-γ⁺IL-2⁺TNF-α⁺ (depicted as g+2+T+); IFN-γ⁺IL-2⁺TNF-α^- (g+2+T–); IFN-γ⁺IL-2^-TNF-α⁺ (g+2–T+); IFN-γ⁺IL-2^-TNF-α^- (g+2–T–); IFN-γ^-IL-2⁺TNF-α⁺ (g-2⁺T⁺); IFN-γ^-IL-2⁺TNF-α^- (g–2+T–) and IFN-γ^-IL-2^-TNF-α⁺ (g–2–T+) among infants and adults. P-values were calculated using the Mann–Whitney U-test; *<0·05; **<0·005; ***<0·0005.

Fig. 3 — Type 1 CD4⁺ T cell response in infants and adults. (a) Flow cytometry dot-plots show co-expression of early T cell activation marker CD69, with various individual cytokines [tumour necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL-2)] among infants and adults that were gated previously on CD4⁺ T cells, as described in Fig. 1a. Frequencies of various T helper type 1 (Th1) cytokine combinations generated with Boolean gating were normalized with total CD4⁺ T cells in the respective samples, background-corrected with unstimulated controls and then presented as a bar graph (described in Methods) Open triangles represent infants while closed triangles represent adults. (b) or pie charts (c) & (d). The bar graphs show mean percentages of CD4⁺ T cells (all IL-4^-) expressing IFN-γ⁺IL-2⁺TNF-α⁺ (depicted as g+2+T+); IFN-γ⁺IL-2⁺TNF-α^- (g+2+T–); IFN-γ⁺IL-2^-TNF-α⁺ (g+2–T+); IFN-γ⁺IL-2^-TNF-α^- (g+2–T–); IFN-γ^-IL-2⁺TNF-α⁺ (g-2⁺T⁺); IFN-γ^-IL-2⁺TNF-α^- (g–2+T–) and IFN-γ^-IL-2^-TNF-α⁺ (g–2–T+) among infants and adults. P-values were calculated using the Mann–Whitney U-test; *<0·05; **<0·005; ***<0·0005.