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Localization of interleukin (IL)-6 in tryptase-positive mast cells in psoriatic skin using the sequential double-staining technique. The skin cryosection was stained first (a,c) enzyme-histochemically for tryptase. After photographing, the red staining product was dissolved away with 15% Tween20 overnight followed by (b,d) staining immunohistochemically for IL-6 and re-photographing. (a,b) Non-lesional psoriatic skin at day 0; (c,d) Köbner-positive psoriatic skin at day 3.
