TABLE 1.

Oligonucleotide primers for multiplex PCR of subgenus C Ads

Serotype	Primer	Polarity	Positiona	Sequence (5′-3′)	Amplicon length (bp)
	AdCF	+	657-677	5′-TGC TTG CGC THA AAA TGG GCA-3′
Ad1	Ad1R	−	895-873	5′-CGA GTA TAA GAC GCC TAT TTA CA-3′	630
Ad2	Ad2R	−	838-857	5′-CGC TAA GAG CGC CGC TAG TA-3′	204
Ad5	Ad5R	−	1093-1111	5′-ATG CAA AGG AGC CCC GTA C-3′	455
Ad6	Ad6R	−	1209-1188	5′-CTT GCA GTC TTT ATC TGA AGC A-3′	929

^aThe positions of primers AdCF and Ad2R are based on the Ad2 fiber gene; that of primer Ad5R is based on the Ad5 fiber gene (6). Ad1R and Ad6R are based on the fiber gene sequences of Ad1 and Ad6, respectively (GenBank accession numbers AB108423 and AB108424, respectively).