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Substrate specificity of OfHex2 for N-glycan and sugar parts of glycosphingolipids with terminal GlcNAc/ GalNAc residues.
| Substrates | Hydrolysis rate a (nmol∙min-1∙μg-1) | Relative activity (%) |
|---|---|---|
| MU-β-GlcNAc | 0.7349±0.0043 | 100 |
| GnGn-PA | 0.03037±0.0008 | 4.132 |
| Gb4-PA | 0.06218±0.0014 | 8.461 |
| GA2-PA | 0.1387±0.0017 | 18.87 |
| GM2-PA | N.D.b | N.D. |
a Enzyme activity was measured at pH 5.5 with 5 μM substrate. Data are the mean ±S.D. of three independent experiments.
b. N.D. indicates not detectable.
