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. 2012 Sep 1;8(8):1097–1108. doi: 10.7150/ijbs.4524

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 5 — Effect of IPTG concentrations and culture temperatures on the transport and surface-binding activities of InaQ-N/GFP. (A) Correlation between IPTG concentration and GFP fluorescence intensity of E. coli JM109/pMB102 expressing the InaQ-N/GFP fusion protein. (B) Residual GFP fluorescence intensity of intact cells after pronase proteolysis. (C) Whole-cell and residual GFP fluorescence intensity of the outer membrane fractions of JM109/pMB102 cells cultured at different temperatures and without/with pronase proteolysis.