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. 2012 Sep 1;8(8):1097–1108. doi: 10.7150/ijbs.4524

Fig 6.

Fig 6

Expression profiles of E. coli JM109/pMB102, JM109/pMB109, and JM109/pMB110 cells expressing InaQ-N/GFP, (InaQ-N)2/GFP and (InaQ-N)3/GFP, respectively. (A) Western blot analysis of the cell fraction samples. Lanes 1 and 4, JM109/pMB102; lanes 2 and 5, JM109/pMB109; lanes 3 and 6, JM109/pMB110. (B) Percentage of specific GFP fluorescence intensities of subcellular fractions. E. coli JM109/pGFPuv expressing cellular GFP was used as the negative control of protein surface-immobilization. In (A) and (B), Sol, soluble cytoplasmic fraction; OM, outer membrane fraction; IM, inner membrane fraction.