Skip to main content
. 2012 Sep 6;8(8):1109–1120. doi: 10.7150/ijbs.4694

Figure 3.

Figure 3

Effect of IFNγ and LNMMA on CL-2, CL-19 and Renca cell proliferation. (A) Three hundred thousand cells from CL-2, CL-19 and Renca were seeded in six well plates and cultured in presence or absence of IFNγ (10U/ml) or iNOS inhibitor LNMMA (1mM). Cell proliferation was measured at 24, 48, and 72 h using the MTT assay. The results are expressed as O.D (570) means ± SE of triplicate determinations from five independent experiments. Significant differences in proliferation were observed between control and IFNγ treated CL-2 (* p= 0.0084) and CL-19 (**p=0.0001) cells respectively. (B) Morphological changes and inhibition of cell proliferation in the CL-2, CL-19 and Renca cells before and after treatment with IFNγ. Microscopic images of mRCC cells after treatment with IFNγ were taken at 48h. Photographs were taken under phase contrast at 100x magnification.