TABLE 1.
Comparison of dematiaceous filamentous fungi identification using conventional methods and nucleic acid sequencing (≤1%)
| Conventional phenotypic identification | MicroSeq sequence-based identification (no. of isolates) | No. of isolates | No. of isolates with concordant resultsa | Distance score avg range |
|---|---|---|---|---|
| Alternaria species | Alternaria alternata (4) | 5 | 4 | 0 |
| Stemphylium/Cochliobolus (1)c | ||||
| Aureobasidium pullulans | Aureobasidium pullulans (1) | 1 | 1 | 0.62 |
| Bipolaris species | Bipolaris spicifera (3) | 3 | 3 | 0.00 |
| Chaetomium species | Chaetomium globosum (4) | 4 | 4 | 0-0.94 |
| Chalara species | Chaetomium globosum (1) | 1 | 0 | 0.94 |
| Cladosporium species | Mycosphaerella tassiana (3)b | 4 | 3 | 0-0.23 |
| Exophiala jeanselmei (1) | ||||
| Curvularia species | Pyrenophora semeniperda (1)b | 1 | 1 | 0 |
| Epicoccum species | Phyllosticta maydis (2) | 2 | 0 | 0.62 |
| Exophiala jeanselmei | Exophiala jeanselmei (1) | 1 | 1 | 0.31 |
| Exserohilum species | Setosphaeria monocerasb-Bipolaris indicia (2) | 2 | 2 | 0-0.62 |
| Pestalotia species | Pestalotia rhododendri (1) | 1 | 1 | 0 |
| Phialophora verrucosa | Phialophora americana (1) | 1 | 1 | 0.93 |
| Pithomyces species | Pithomyces atro-olivaceus (2) | 2 | 2 | 0-0.65 |
| Scedosporium prolificans | Petriella boulangeri (4)b | 4 | 4 | 0.94 |
| Scedosporium apiospermum | Pseudallescheria ellipsoidea (1)b | 1 | 1 | 0 |
| Scytalidium species | Scytalidium hyalinum (1) | 2 | 1 | 0 |
| Phyllosticta maydis (1) | ||||
| Sporothrix species | Hypopichia burtonii (1)b | 1 | 0 | 0 |
| Sporotrichum pruinosum | Phanerochaete chrysosporium (1)b | 1 | 1 | 0.57 |
| Ulocladium species | Ulocladium chartarum (1) | 2 | 1 | 0-0.031 |
| Allewia eureka (1) |
a
Sequence identification concordant with genus or genus and species.
b
Teleomorph.
c
The MicroSeq system could not distinguish between the two genera listed.