TABLE 3.
Comparison of hyaline filamentous fungi identification using conventional methods and nucleic acid sequencing (≤1%)
| Conventional phenotypic identification | MicroSeq sequence-based identification (no. of isolates) | No. of isolates | No. of isolates with concordant resultsa | Distance score avg range |
|---|---|---|---|---|
| Aspergillus flavus | Aspergillus ochraceous (1) | 1 | 1 | 0.62 |
| Aspergillus fumigatus | Neosartorya pseudofischeri (10) | 10 | 0 | 0.31 |
| Aspergillus glaucus | Eurotium-Setosphaeria-Bipolaris (4) | 4 | 0 | 0 |
| Aspergillus nidulans | Emericella nidulans (1)b | 4 | 1 | 0.16-0.55 |
| Emericella parvathecia (3) | ||||
| Aspergillus niger | Aspergillus niger/Aspergillus foetidus (5)c | 8 | 7 | 0-0.93 |
| Aspergillus niger/Aspergillus awamori (2)c | ||||
| Aspergillus species (1) | ||||
| Aspergillus species | Aspergillus candidus (1) | 4 | 4 | 0-0.31 |
| Aspergillus sclerotiorum (1) | ||||
| Aspergillus carneus (1) | ||||
| Emericella parvathecia (1)b | ||||
| Aspergillus terreus | Aspergillus carneus (7) | 7 | 0 | 0.31 |
| Aspergillus versicolor | Aspergillus versicolor (4) | 4 | 4 | 0-0.93 |
| Fusarium species | Fusarium solani (6) | 9 | 9 | 0-0.62 |
| Fusarium chlamydosporum (2) | ||||
| Fusarium verticillioides (1) | ||||
| Geomyces species | Geomyces pannorum (1) | 1 | 1 | 0 |
| Geotrichum species | Leptosphaerulina australis (2) | 2 | 0 | |
| Histoplasma capsulatum | Histoplasma capsulatum (10) | 10 | 10 | 0-0.31 |
| Mucor species | Mucor racemosus (1) | 2 | 2 | 0-0.23 |
| Mucor circinelloides (1) | ||||
| Onychocola canadensis | Filobasidium uniguttulatum (1) | 1 | 0 | 0.50 |
| Paecilomyces lilacinus | Paecilomyces lilacinus (4) | 4 | 4 | 0-0.31 |
| Penicillium marneffei | Penicillium rubrum (1) | 1 | 0 | 0.93 |
| Penicillium species | Penicillium chrysogenum (2) | 6 | 6 | 0-0.31 |
| Penicillium rubrum (2) | ||||
| Penicillium clavigerum (1) | ||||
| Penicillium olsonii (1) | ||||
| Rhizopus species | Rhizopus oryzae (1) | 1 | 1 | 0.5 |
| Rhizopus stolonifera | Rhizopus stolonifera (1) | 1 | 1 | 0 |
| Scopulariopsis species | Scopulariopsis koningii (4) | 4 | 4 | 0 |
| Trichoderma species | Trichoderma inhamatum (2) | 4 | 4 | 0-0.94 |
| Trichoderma koningii (1) | ||||
| Trichoderma harzianum (1) |
a
Sequence identification concordant with genus or genus and species.
b
Teleomorph.
c
The MicroSeq system could not distinguish between the two species listed.