TABLE 4.
Comparison of hyaline filamentous fungi identification using conventional methods and nucleic acid sequencing (≥1%)
| Conventional phenotypic identification | MicroSeq sequence-based identification (no. of isolates) | No. of isolates | No. of isolates with concordant resultsa | Distance score avg range |
|---|---|---|---|---|
| Absidia species | Zygorhynchus moelleri (5) | 6 | 0 | 24.54-26.7 |
| Mucor flavus (1) | ||||
| Apophysomyces elegans | Zygorhynchus moelleri (2) | 2 | 0 | 25.23 |
| Aspergillus clavatus | Neosartorya pseudofischeri (1) | 1 | 0 | 1.56 |
| Aspergillus flavus | Aspergillus foetidus/Aspergillus niger (6)c | 6 | 0 | 3.12 |
| Beauveria species | Beauveria bassiana (1) | 1 | 1 | 2.20 |
| Blastomyces dermatitidis | Histoplasma capsulatum (8) | 8 | 0 | 1.55 |
| Chrysosporium species | Malbranchea (1) | 3 | 0 | 7.81-8.39 |
| Malbranchea pulchella (1) | ||||
| Gymnoascus reesii (1) | ||||
| Coccidioides immitis | Chrysosporium queenslandicum (4) | 4 | 0 | 4.04 |
| Cokeromyces recurvatus | Cokeromyces recurvatus (1) | 1 | 1 | 2.05 |
| Cunninghamella species | Pichia pastoris (1) | 1 | 0 | 46.20 |
| Delacroixa coronata | Conidiobolus incongruous (1)b | 1 | 1 | 20.76 |
| Fugomyces cyanescens | Rhodotorula bacarum (3) | 3 | 0 | 5.17 |
| Fusarium species | Fusarium oxysporum (1) | 1 | 1 | 1.25 |
| Geotrichum klebhanii | Geotrichum candidum, citri-aurantii (2) | 2 | 0 | 6.39 |
| Blastoschizomyces capitatus | Geotrichum fragrans (1) | 1 | 0 | 4.81 |
| Gonytrichum species | Chaetomium brasiliensis (1) | 1 | 0 | 11.29 |
| Gymnascella citrina | Penicillium rubrum (1) | 1 | 0 | 8.07 |
| Malbranchea species | Malbranchea pulchella (2) | 2 | 2 | 3.41-8.36 |
| Mucor species | Mucor hiemalis (1) | 3 | 2 | 1.76-48.44 |
| Mucor circinelloides (1) | ||||
| Aplanopsis spinosa (1) | ||||
| Myriodontium species | Arachnotheca albicans (1) | 1 | 0 | 7.48 |
| Oidiodendron species | Oidiodendron tenuissimum (1) | 1 | 1 | 2.80 |
| Paecilomyces lilacinus | Paecilomyces lilacinus (1) | 1 | 1 | 4.40 |
| Paecilomyces species | Paecilomyces puntonii (2) | 3 | 3 | 1.86-3.73 |
| Paecilomyces variotii (1) | ||||
| Penicillium marneffei | Penicillium rubrum (1) | 1 | 0 | 6.68 |
| Penicillium species | Penicillium rubrum (3) | 4 | 4 | 1.24-1.55 |
| Penicillium solitum (1) | ||||
| Rhizomucor miehei | Rhizomucor miehei (1) | 1 | 1 | 3.50 |
| Rhizopus species | Aplanopsis spinosa (1) | 1 | 0 | 47.68 |
| Sepedonium species | Mycogone perniciosa (1) | 1 | 0 | 4.08 |
| Spegazzinia species | Pithomyces atro-olivaceus (1) | 1 | 0 | 6.83 |
| Syncephalastrum racemosum | Syncephalastrum racemosum (1) | 1 | 1 | 2.17 |
| Syncephalastrum species | Syncephalastrum (1) | 1 | 1 | 5.02 |
| Trichothecium roseum | Stilbella albocitrina (1) | 1 | 0 | 2.20-2.52 |
a
Sequence identification concordant with genus or genus and species.
b
Telemorph.
c
The MicroSeq system could not distinguish between the two species listed.