Phylogenetic tree of the intimin variant genes constructed with the CLUSTAL W program. Numbers on the branches denote the genetic distance. Phylogenetic analyses revealed five groups of the closely related intimin genes: (i) α1, α2, ζ, and ν; (ii) β1, β2, δ, and κ; (iii) γ1, γ2, θ, and μ; (iv) ι and λ; and (v) ɛ, η, and ξ.