TABLE 2.
NCCLS broth microdilution MICs as reported by three laboratories for 100 clinical isolates of Candida spp. and percent agreement between YeastOne colorimetric and NCCLS MICs
| Fungus (no. of isolates tested) | Antifungal agent | Reference MIC range (μg/ml) by reference M27-A2 methoda | MIC50/MIC90b (μg/ml) | % Agreement between YeastOne and M27-A2 MIC pairs at incubation times (h) of:
|
|
|---|---|---|---|---|---|
| 24 vs 24 | 24 vs 48 | ||||
| C. albicans (38) | Caspofungin | 0.03-1 | 0.12/0.5 | 99 | 97 |
| Posaconazole | 0.03->8 | 0.12/1 | 95 | 71 | |
| Ravuconazole | 0.008->8 | 0.06/1 | 97 | 82 | |
| Voriconazole | 0.008->8 | 0.06/2 | 99 | 86 | |
| Fluconazole | 0.12->64 | 1/>64 | 99 | 90 | |
| Amphotericin B | 0.5-8 | 1/2 | 99 | 98 | |
| C. glabrata (24) | Caspofungin | 0.06-2 | 0.25/1 | 99 | 94 |
| Posaconazole | 0.12->8 | 2/4 | 97 | 87 | |
| Ravuconazole | 0.03->8 | 1/4 | 99 | 84 | |
| Voriconazole | 0.03-4 | 1/4 | 99 | 93 | |
| Fluconazole | 1->64 | 32/64 | 96 | 93 | |
| Amphotericin B | 0.5-2 | 1/2 | 100 | 99 | |
| C. krusei (7) | Caspofungin | 0.25-2 | 1/1 | 95 | 100 |
| Posaconazole | 0.25-1 | 0.5/1 | 100 | 100 | |
| Ravuconazole | 0.12-1 | 0.5/1 | 100 | 100 | |
| Voriconazole | 0.25-1 | 0.5/1 | 100 | 100 | |
| Fluconazole | 8->64 | 64/64 | 100 | 95 | |
| Amphotericin B | 1-4 | 2/4 | 100 | 100 | |
| C. lusitaniae (5) | Caspofungin | 0.25-1 | 1/1 | 93 | 87 |
| Posaconazole | 0.06-0.25 | 0.12/0.12 | 87 | 73 | |
| Ravuconazole | 0.008-0.12 | 0.03/0.06 | 100 | 100 | |
| Voriconazole | 0.008-0.03 | 0.016/0.03 | 100 | 100 | |
| Fluconazole | 0.25-2 | 1/1 | 100 | 100 | |
| Amphotericin B | 0.5-2 | 1/2 | 93 | 87 | |
| C. parapsilosis (10) | Caspofungin | 0.25-2 | 1/2 | 100 | 93 |
| Posaconazole | 0.06-0.5 | 0.12/0.25 | 100 | 97 | |
| Ravuconazole | 0.016-0.25 | 0.12/0.25 | 93 | 97 | |
| Voriconazole | 0.016-0.25 | 0.06/0.25 | 100 | 100 | |
| Fluconazole | 0.5-16 | 4/8 | 100 | 100 | |
| Amphotericin B | 1-2 | 1/2 | 100 | 97 | |
| C. tropicalis (16) | Caspofungin | 0.03-16 | 0.06/1 | 89 | 92 |
| Posaconazole | 0.03-1 | 0.25/0.5 | 98 | 92 | |
| Ravuconazole | 0.008-1 | 0.06/0.25 | 85 | 76 | |
| Voriconazole | 0.016-0.5 | 0.06/0.5 | 96 | 91 | |
| Fluconazole | 0.25-8 | 1/4 | 96 | 94 | |
| Amphotericin B | 0.5-4 | 2/4 | 98 | 100 | |
| All isolates (100) | Caspofungin | 0.03-16 | 0.25/1 | 96 | 94 |
| Posaconazole | 0.03-4 | 0.25/2 | 96 | 87 | |
| Ravuconazole | 0.008->8 | 0.12/2 | 95 | 80 | |
| Voriconazole | 0.008->8 | 0.12/2 | 98 | 95 | |
| Fluconazole | 0.12->64 | 1/>64 | 99 | 95 | |
| Amphotericin B | 0.5-8 | 1/2 | 98 | 97 | |
a
Data are combined MIC data from the three centers, representing 15 to 114 MICs per species.
b
MIC50 is the MIC at which 50% of the strains are inhibited; MIC90 is the MIC at which 90% of the strains are inhibited.