Fig. 4.

fbf-1 affects the distribution of meiotic mRNAs in the distal gonad. (A) Distal gonads of the indicated genotypes hybridized to fluorescent probes specific for gld-1, htp-1/2, him-3 and tbb-2 mRNAs. Germlines are outlined; vertical dotted lines indicate the transition zone as recognized by DAPI staining (not shown). (B) The percentage of germlines with aggregates of the indicated mRNAs as visualized by in situ hybridization as shown in A. N, the number of gonads scored for each genotype and mRNA. (C) fbf-1 mutant distal gonad double stained for GFP::FBF-2 and gld-1 mRNA. Arrow points to a cytoplasmic aggregate that is positive for both GFP::FBF-2 and gld-1 mRNA.