Stage-dependent effects of Dynein on muscle length and myonuclear positioning. (A) Immunofluorescence images of the muscles (green, Tropomyosin) and nuclei (red, DsRed) at stage 14, 15 and 16 in control and Dhc64C4-19 Drosophila embryos. Scale bar: 10 μm. (B) The length of the muscles in control (gray) and Dhc64C4-19 (black) embryos at stage 14, 15 and 16. (C-E) The distance between the nearest nucleus and either the dorsal (gray) or ventral (white) pole of the muscle in stage 14 (C), 15 (D) and 16 (E) embryos. Error bars indicate s.d.; *P<0.05, **P<0.01.