The pathways controlling muscle length and myonuclear position do not genetically interact. (A) Immunofluorescence images of stage 16 Drosophila embryos that are doubly heterozygous for the indicated alleles. Green, Tropomyosin; red, DsRed. Scale bar: 10 μm. (B) LT muscle length in stage 16 embryos that are doubly heterozygous for the indicated alleles. (C) The shortest distance between the indicated pole of the LT muscles (gray, dorsal; white, ventral) and the nearest cluster of nuclei in stage 16 embryos normalized for muscle length. Error bars indicate s.d.; *P<0.05, **P<0.01.