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. 2012 Aug 1;125(15):3535–3544. doi: 10.1242/jcs.097105

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© 2012. Published by The Company of Biologists Ltd

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Fig. 6. — Elevated cyclin E activity is not required for suppression of zyg-1(it25) by SEL-10 depletion. (A) Western blot comparing CYE-1 levels between control and sel-10(ok1632) embryos and showing successful depletion of CYE-1 under our RNAi conditions. (B) A representative embryo from zyg-1(it25) fertilized, feminized zyg-1(it25); gfp::spd-2; fem-1(hc17); sel-10(ok1632); cye-1(RNAi) worms grown at 24°C. Arrowheads indicate centrosomes. (C) Quantification of the percentage of centrosomes that duplicated during the first cell cycle in control and CYE-1 depleted embryos. Control, n = 35; cye-1(RNAi), n = 17.