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. 2012 Aug 1;125(15):3630–3635. doi: 10.1242/jcs.102889

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use, distribution and reproduction in any medium providedthat the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 1. — SUMOylation during human embryonic stem cell self-renewal and hepatocyte differentiation. (A,B) Cell extracts were collected at various time points throughout the differentiation procedure (days 0–17) and separated by SDS-PAGE. Western blots were probed for SUMO1, SUMO2, SENP7, hepatocyte nuclear factor 4 α (HNF4α), albumin (ALB) and β-actin. A decrease in SUMO-modified proteins is observed as the cells commit to hepatic endoderm. (C) During stem cell differentiation to hepatocytes we observed an increase in levels of the SUMO-deconjugating enzyme SENP7 and the serum protein albumin. We detected HNF4α expression from day 7, which decreased at days 15 and 17. (D) Day 17 hepatocytes were fixed and stained for DNA (A), HNF4α (B), albumin (C) and overlaid (D). The percentage of positive cells (shown top right on each panel) was calculated from five random fields of view and are quoted ± 1 s.d.