Table II.
Mass spectrometric identification of spots on two-dimensional gels
| Spot No. | Database | Gene Identifier | Annotation | MOWSE (Molecular Weight Search) Score | Peptides Matched (Total No. of Peptides) | Predicted Molecular Mass (D) | Predicted pl | Coveragea | Codine Site | Plastid Targetingb |
|---|---|---|---|---|---|---|---|---|---|---|
| % | ||||||||||
| 3317 | National Center for Biotechnology Information (NCBI) | 134102 | 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor | 5.42 E + 03 | 7 (32) | 57,521 | 4.83 | 13 | Nucleus | Yes |
| 3603 | NCBI | 134102 | 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor | 5.42 E+03 | 7 (32) | 57,521 | 4.83 | 13 | Nucleus | Yes |
| 3610 | NCBI | 134102 | 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor | 1.00 E+04 | 7 (22) | 57,521 | 4.83 | 16 | Nucleus | Yes |
| 3615 | NCBI | 134102 | 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor | 1.60 E+05 | 9 (31) | 57,521 | 4.83 | 18 | Nucleus | Yes |
| 3324 | The Institute for Genomic Research (TIGR) Zm | TC88712 | 20-kD chaperonin (Cpn20), chloroplast precursor | 9.88 E+5 | 8 (25) | 27,095 | 6.25 | 37 | Nucleus | Yes |
| 4209 | TIGR Zm | TC88576* | 20-kD chaperonin (Cpn20), chloroplast precursor | 1.02 E+7 | 8 (19) | 26,569 | 8.48 | 45 | Nucleus | Yes |
| 3320 | NCBI | 15912247 or 13926292 | 33-kD subunit of OEC, PSI | 93.4 | 4 (22) | 35,128 | 5.55 | 11 | Nucleus | Yes |
| 3330 | NCBI | 15408655 | 33-kD subunit of OEC, PSI (pulative) | 984 | 4 (25) | 34,861 | 6.09 | 17 | Nucleus | Yes |
| 4305 | TIGR Zm | TC81083 | 33-kD subunit of OEC, PSII | 2.13 E+6 | 9 (26) | 37,531 | 5.92 | 43 | Nucleus | Yes |
| 6537 | TIGR Zm | TC88557 | Acetyl CoA carboxylase | 214 | 5 (22) | 258,314 | 5.93 | 2 | Nucleus | Yes |
| 7537 | NCBI | 7438099 | Acetyl CoA carboxylase | 1.25 E+3 | 9 (32) | 252,131 | 5.91 | 6 | Nucleus | No |
| 7616 | TIGR Zm | TC90687 | ATPase subunit alpha | 5.48 E+5 | 8 (31) | 55,707 | 5.86 | 23 | Plastid | N.A.c |
| 8632 | TIGR Zm | TC90687 | ATPase subunit alpha | 1.36 E+5 | 8 (26) | 55,707 | 5.86 | 19 | Plastid | N.A. |
| 8633 | TIGR Zm | TC90687 | ATPase subunit alpha | 2.87 E+5 | 7 (30) | 55,707 | 5.86 | 19 | Plastid | N.A. |
| 1246 | TIGR Zm | TC85581 | ATPase subunit beta | 1.69 E+7 | 11 (29) | 54,041 | 5.31 | 28 | Plastid | N.A. |
| 4520 | NCBI | 629818 | ATPase subunit beta | 7.15 E+4 | 7 (16) | 59,249 | 5.56 | 18 | Plastid | N.A. |
| 4533 | NCBI | 552857 | ATPase subunit beta | 5.82 E+5 | 9 (23) | 53,954 | 5.30 | 19 | Plastid | N.A. |
| 4534 | NCBI | 629818 | ATPase subunit beta | 1.54 E+5 | 7 (14) | 59,249 | 5.56 | 20 | Plastid | N.A. |
| 5504 | NCBI | 552857 | ATPase subunit beta | 3.52 E+3 | 5 (24) | 53,954 | 5.30 | 17 | Plastid | N.A. |
| 5507 | NCBI | 629818 | ATPase subunit beta | 7.23 E3 | 6 (22) | 59,249 | 5.56 | 14 | Plastid | N.A. |
| 5517 | NCBI | 6815115 | ATPase subunit beta | 4.63 E+5 | 11 (30) | 53,997 | 5.38 | 23 | Plastid | N.A. |
| 5518 | NCBI | 629818 | ATPase subunit beta | 1.14 E+3 | 5 (19) | 59,149 | 5.56 | 11 | Plastid | N.A. |
| 5725 | NCBI | 874823 | ATPase subunit beta | 4.86 E+4 | 7 (32) | 53,717 | 5.20 | 18 | Plastid | N.A. |
| 6511 | NCBI | 552857 | ATPase subunit beta | 2.54 E+5 | 9 (19) | 53,954 | 5.30 | 24 | Plastid | N.A. |
| 6708 | TIGR Zm | TC85581 | ATPase subunit beta | 3.69 E+6 | 12 (28) | 54,041 | 5.31 | 30 | Plastid | N.A. |
| 201 | TIGR Zm | TC86875 | ATPase subunit delta | 1.22 E+4 | 5 (17) | 20,206 | 4.35 | 38 | Nucleus | Yes |
| 6439 | TIGR Zm | TC88574 | ATPase subunit gamma | 104 | 4 (17) | 42,529 | 8.80 | 11 | Nucleus | Yes |
| 7414 | TIGR Zm | TC88574 | ATPase subunit gamma | 1.21 E+3 | 7 (14) | 42,529 | 8.83 | 18 | Nucleus | Yes |
| 8520 | NCBI | 7489718 | Beta-D-glucosidase, glu2 precursor | 1.74 E+2 | 7 (16) | 64,112 | 6.72 | 16 | Nucleus | Yes |
| % | ||||||||||
| 5406 | TIGR Zm | TC85027 | Chloroplast NADP-malate dehydrogenase | 9.64E+3 | 6 (30) | 49,481 | 6.62 | 17 | Nucleus | Yes |
| 6705 | NCBI | 5360574 | ClpC protease | 5.16E+3 | 6 (19) | 105,740 | 6.06 | 8 | Nucleus | Yes |
| 6711 | NCBI | 2921158 | ClpC protease | 3.95E+7 | 14 (26) | 103,456 | 6.27 | 14 | Nucleus | Yes |
| 3632 | NCBI | 16444957 | Cryptochrome 1 | 1.05E+3 | 5 (20) | 79,300 | 5.37 | 9 | Nucleus | No |
| 4738 | NCBI | 16444957 | Cryptochrome 1 | 631 | 4 (26) | 79,301 | 5.37 | 8 | Nucleus | No |
| 4741 | NCBI | 16444957 | Cryptochrome 1 | 2.63E+3 | 5 (32) | 79,300 | 5.37 | 10 | Nucleus | No |
| 4417 | NCBI | 15809970 | Enolase (2 phospho D glycerate hydroylase) | 1.47E+3 | 5 (31) | 47,704 | 5.74 | 18 | Nucleus | No |
| 2714 | NCBI | 6746592 | Hsp70 | 9.32E+3 | 7 (33) | 77,106 | 5.13 | 10 | Nucleus | Yes |
| 2720 | NCBI | 6746592 | Hsp70 | 5.55E+4 | 8 (27) | 77,106 | 5.13 | 11 | Nucleus | Yes |
| 329 | NCBI | 100903 | Nucleic acid-binding protein (NABP) | 1.51E+3 | 4 (24) | 33,117 | 4.60 | 18 | Nucleus | Yes |
| 4409 | TIGR Zm | TC82882 | Phosphoglycerate kinase, chloroplast precursor | 2.44E+4 | 5 (28) | 24,114 | 9.12 | 33 | Nucleus | Yes |
| 4219 | TIGR Zm | TC89102 | Plastid-specific ribosomal protein “2” | 1.08E+5 | 8 (17) | 26,232 | 8.46 | 25 | Nucleus | Yes |
| 5421 | NCBI | 13489165 | Beta amylase | 3.37E+3 | 8 (32) | 57,953 | 6.74 | 19 | Nucleus | Yes |
| 6441 | TIGR Zm | TC82049 | SU1 isoamylase | 468 | 4 (21) | 91,619 | 5.85 | 6 | Nucleus | Yes |
| 7313 | TIGR Zm | TC81138 | Sucrose synthase (UDP-glucose: D fructose-2-glucosyl-transferase) | 152 | 4 (9) | 93,696 | 6.18 | 4 | Nucleus | No |
| 6402 | TIGR Zm | TC84809 | Glyceraldehyde-3-phosphate dehydrogenase | 3.62E+4 | 5 (25) | 46,952 | 5.95 | 18 | Nucleus | Yes |
| 3629 | NCB1 | 12325133 | Unknown protein | 193 | 6 (25) | 66,659 | 5.06 | 12 | Nucleus | No |
| 7230 | NCB1 | 16905193 | Hypothetical protein | 3.09E+2 | 4 (23) | 26,072 | 6.34 | 21 | Nucleus | No |
| 1353 | NCBI | 7459088 | Hypothetical protein | 4.05E+3 | 4 (37) | 11,782 | 4.94d | 28 | Nucleus | No |
| 3331 | NCBI | 16604341 | Unknown protein | 2.36E+3 | 5 (20) | 39,424 | 5.12d | 13 | Nucleus | No |
| 1145 | TIGR Zm | TC81772 | Inosinc monophosphate dehydrogenase | 3.65E+4 | 6 (26) | 11,784 | 9.78d | 61 | Nucleus | No |
| 2528 | NCBI | 3915111 | Cytochrome P450 (C4H) | 1.5 E+3 | 5 (23) | 58,011 | 9.05d | 16 | Nucleus | No |
| 3602 | NCBI | 15554512 | Male sterility 1 protein | 1.40 E14 | 7 (32) | 76,974.2 | 7.82d | 17 | Nucleus | No |
| 4301 | TIGR Zm | TC89587 | Mitotic spindle checkpoint protein MAD2 | 2.61 E4 | 4 (21) | 26,664 | 4.73d | 24 | Nucleus | No |
| 6217 | TIGR Zm | TC89879 | Putative protein phosphatase | 1.03E+3 | 4 (37) | 25,946 | 10.38d | 25 | Nucleus | No |
a
Percent of predicted protein sequence the peptides matched. b Plastid targeting sequence determined by ChloroP software. c N.A., Not applicable, protein is plastid encoded. d Predicted pl does not match the pl of the spot on the gel.